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Combining site-directed mutations and reconstitution with a locked chromophore analog converts photoactive yellow protein into a highly fluorescent protein tag

BACKGROUND Fluorescent protein tags, such as green fluorescent protein (GFP), exhibit fluorescence when viewed under ultraviolet light. Since these tags make it possible to 'see' metabolic interactions, attaching them to proteins, antibodies, and amino acids allows researchers to study interactions between biomolecules and thus improve our...

Published: 10/19/2022 | Inventor(s): Masato Kumauchi, Branden Carr, Wouter Hoff

Keywords(s): Research Tools & Methods

Category(s): Technology Classifications > Research Tools