Fluorescent protein tags, such as green fluorescent protein (GFP), exhibit fluorescence when viewed under ultraviolet light. Since these tags make it possible to 'see' metabolic interactions, attaching them to proteins, antibodies, and amino acids allows researchers to study interactions between biomolecules and thus improve our understanding of such diverse biological processes as those involved in brain functionality, specific protein-protein interactions, and the spread of disease. Although GFP is responsible for many advances in modern research and remains in use today, its utility is limited by the fact that it is relatively large (26kD), has a slow reaction time, and requires oxygen to function.
SUMMARY OF TECHNOLOGY
OSU researchers have developed a method of converting photoactive yellow protein (PYP) into a usable fluorescent protein tag. PYP is half the size (~14kD) and has a faster reaction time than GFP and other fluorescent proteins currently available. Most importantly, PYP does not require oxygen, making it ideal for use in anaerobic studies such as tumor research. Not only does the development of a new fluorescent protein tag open up new research opportunities, but this new technique may permit the transformation of proteins that are closely related to PYP into usable fluorescent protein tags.
POTENTIAL AREAS OF APPLICATION
MAIN ADVANTAGES
STATE OF DEVELOPMENT
Proof of concept stage.