Inhibitors of the BfrB-Bfd Complex for Killing Biofilm Bacteria

Case ID:
2020-030

­BACKGROUND

Biofilms are communities of microbes adhering to a surface and often embedded in an extracellular matrix. As a result of this structure, biofilms exhibit resistance to both host immune responses and antibiotics. Approximately 80% of human bacterial infections involve biofilms; this can take the form of chronic wound infections, chronic otitis media, osteomyelitis, recurrent urinary tract infection, endocarditis, and cystic fibrosis-associated lung infections. There is also a high incidence of biofilm-associated infections affecting indwelling catheters, orthopedic implants, stents and implantable electronic devices. Consequently, there is an urgent need for innovative therapeutic approaches to treat biofilm infections.

SUMMARY OF TECHNOLOGY

Researchers at OSU and LSU have developed synthetic methods to prepare molecules that inhibit the bacterioferritin (BfrB) and bacterioferritin-associated ferredoxin (Bfd). Inhibiting this protein complex formation disrupts the iron-homeostasis, causing an irreversible accumulation of Fe3+ in BfrB, accompanied by cytosolic iron deficiency. A major benefit of disrupting this system is that BfrB-Bfd are only found in bacteria, making this system theoretically unable to damage eukaryotic cells. Test data show efficacy at killing bacteria in both planktonic and biofilm settings.

POTENTIAL AREAS OF APPLICATION

  • Antibiotics
  • Topical antibiotics
  • Antibiotic potentiators
  • Wound dressing

MAIN ADVANTAGES

  • Kills bacteria instead of preventing reproduction
  • Effective at impairing biofilm growth
  • Kills bacteria in mature biofilms
  • Only effects bacteria

STAGE OF DEVELOPMENT

  • Lead candidates have been tested using in-vitro assays of BfrB-Bfd complex and Pseudonomas aeruginosa
Patent Information:
For Information, Contact:
Russell Hopper
Sr. Licensing Associate
Oklahoma State University
russell.hopper@okstate.edu
Inventors:
Huili Yao
Anabel Soldano
Scott Lovell
Richard Bunce
Mario Rivera
Keywords:
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