Single Step Metagenome Sequencing based Detection of SARS-CoV-2

Case ID:
2021-037

BACKGROUND

Traditional detection of SARS-CoV-2 has primarily relied on real time Reverse Transcription Polymerase Chain Reactions (RT-PCR), a highly sensitive and specific method for identifying viral RNA. This technique is typically conducted in laboratory environments and can require several days to obtain results. Given the rapid transmission of SARS-CoV-2, timely diagnosis is critical for effective infection control and outbreak management. While RT-PCR remains the current standard for COVID-19 diagnosis, there is a growing need for faster, portable and more accessible workflows to help manage the spread of infectious diseases.

SUMMARY OF TECHNOLOGY

Developed by researchers at Oklahoma State University, this technology harnesses the power of genetic e-probes—short, uniquely designed nucleic acid sequences that have been specifically crafted to detect SARS-CoV-2 with high precision. These proprietary e-probes are engineered to function exclusively within OSU’s specialized software, providing ultra-rapid and highly specific detection of the virus directly from sequencing data. The e-probe approach circumvents the need for large reference databases and minimizes computational requirements, enabling clear, actionable results within 5-15 minutes. Optimized for use with portable sequencing devices, this field-ready workflow brings point-of-care molecular diagnostics to virtually any location where it is needed, streamlining pandemic response and safeguarding public health.

POTENTIAL AREAS OF APPLICATION

  • Human Pathogen Detection
  • Nanopore Sequencing Technologies
  • Hospital Laboratory Services
  • Pandemic Preparedness Organizations
  • Biodefense Monitoring Agencies

MAIN ADVANTAGES

  • Single-Step SARS-CoV-2 Detection
  • Streamlined Process Using Specific E-Probes
  • Compatible with Portable Sequencing Devices
  • Effective & Accurate Surveillance Strategies
  • Compatible with Portable Sequencing Devices

STAGE OF DEVELOPMENT

  • Working Model

 

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Patent Information:
For Information, Contact:
Jai Hariprasad Rajendran
Commercialization Officer
Oklahoma State University
jair@okstate.edu
Inventors:
Jerry Malayer
Sai Narayanan
Kitty Cardwell
Andres Espindola Camacho
Akhilesh Ramachandran
Keywords:
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