Single Step, Multiplexed, Metagenome Sequencing based Bacterial Pathogen Detection for Gastrointestinal Disturbances (Salmonella spp., C. perfringens, E. coli, Klebsiella spp.)

BACKGROUND

Gastrointestinal disturbances can be caused by a wide range of pathogens or an overabundance of certain microbial families. Detecting pathogenic bacteria and analyzing the overall microbial composition of the gut can help identify imbalances that may lead to dysbiosis. Current diagnostic technologies often struggle to detect more than a handful of pathogens at once, which is insufficient given the dense and diverse population of the gut microbiome.

SUMMARY OF TECHNOLOGY

Oklahoma State University researchers have created a quick and efficient workflow for gastrointestinal pathogen detection, MiFi. MiFi represents a significant advancement in pathogen detection, employing in-silico probes within metagenomic sequencing to enable rapid, accurate, and simultaneous detection of multiple pathogens, including bacteria like Salmonella and E. coli. This technology sequences the entire genomic content of a sample – whether veterinary, medical -offering a comprehensive approach to pathogen detection. With results available in as little as 5 to 15 minutes, this process is ideal for field and farm applications.

POTENTIAL AREAS OF APPLICATION

• Veterinary & Human Pathogen Detection
• Biodefense & Pathogen Surveillance
• Livestock Health Management
• Wildlife Disease Monitoring

MAIN ADVANTAGES

• Rapid Pathogen Detection
• Multiplexing Capabilities
• Cross-Disciplinary Applications
• Specific Pathogen Detection Using E-Probes

STAGE OF DEVELOPMENT

• Working Model